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Global DNA Methylation ELISA Kit

The Global DNA Methylation ELISA Kit from Cell Biolabs is a highly sensitive competitive ELISA for 5’-methyl-2’-deoxycytidine (5MedCyd) quantification. Validated for use directly with urine or with extracted cell or tissue DNA samples, this kit can rapidly detect and quantify 5MedCyd from a range of species.

Benefits of using the Global DNA Methylation ELISA Kit
• 50 nM 5MedCyd detection sensitivity
• Non labour-intensive procedure
• Compatible with a wide range of species

How it works
The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRPconjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

5MedCyd ELISA standard curve (left) and 5MedCyd levels in various dilutions of human urine (right).

5MedCyd ELISA standard curve (left) and 5MedCyd levels in various dilutions of human urine (right).

Material available for download
Global DNA Methylation ELISA Kit manual

Global DNA Methylation ELISA Kit citation list

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation) STA-380 · Cell Biolabs
STA-380
Cell Biolabs
96 assays £660.00
96 assays
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Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation) STA-380-5 · Cell Biolabs
STA-380-5
Cell Biolabs
5 x 96 assays £2841.00
5 x 96 assays
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