The Global DNA Methylation ELISA Kit from Cell Biolabs is a highly sensitive competitive ELISA for 5’-methyl-2’-deoxycytidine (5MedCyd) quantification. Validated for use directly with urine or with extracted cell or tissue DNA samples, this kit can rapidly detect and quantify 5MedCyd from a range of species.
Benefits of using the Global DNA Methylation ELISA Kit
• 50 nM 5MedCyd detection sensitivity
• Non labour-intensive procedure
• Compatible with a wide range of species
How it works
The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRPconjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.
5MedCyd ELISA standard curve (left) and 5MedCyd levels in various dilutions of human urine (right).