The Genomic DNA Clean and Concentrator™ (DCC) kit from Zymo Research is a gDNA clean up kit that provides rapid recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage or WGA DNA) from any enzymatic reaction or impure preparation (e.g. proteinase K digestion).
Eluted DNA is ideal for downstream applications including sequencing, PCR, endonuclease digestion, microarray and transfection.
Benefits of using the Genomic DCC
• Concentrated DNA eluted in ≥ 10 µl of buffer
• For fragments from 50bp – 200kb (smaller DNA fragments and gDNA)
• 70 – 95% DNA recovery
• 5-minute procedure
Genomic DCC kit workflow
Five minute Genomic DCC-10 procedure.
High molecular weight DNA is efficiently purified using the Genomic DCC
Porcine gDNA (~35-50 kb), T4 phage DNA (170 kb), and lambda phage DNA (48.5 kb) were purified (in duplicate) from input material using the Genomic DCC. Eluted DNAs were analysed in a 0.8% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).
Note: product availability depends on country - see product detail page.