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Protein solubility and expression screening system: Expresso

Lucigen's all-in-one system for protein solubility screens enables PCR amplification, rapid cloning, and expression of your protein of interest with a diverse panel of powerful and cleavable solubility tags. Containing seven fusion tags proven to increase protein expression and solubility, the Expresso® solubility and expression screening system allows you to multiplex your protein solubility screen in order to find the optimal tag.

Benefits of using the Expresso® solubility and expression screening system
• One amplicon to simultaneously generate all your constructs
• Rapid and simultaneous protein screening with TEV-cleavable fusion tags
• Four days from PCR to protein

Expresso solubility and expression screening system workflow

Expresso® Solubility and Expression Screening System Workflow

Expresso® Solubility and Expression Screening System expression vectors

Expresso® Solubility and Expression Screening System Expression Vectors

Each pSOL vector contains everything you need to seamlessly clone in your target gene without any additional amino acids added. Fusion tags are cleavable via a TEV protease site.

GH1 (Human Growth Hormone) was evaluated for E. coli expression and solubility when expressed as a fusion

GH1 (Human Growth Hormone) Was Evaluated For E. coli Expression & Solubility When Expressed As A Fusion

Fusion tags SlyD, Tsf, SUMO, Bla, and MBP demonstrated enhanced solubility (Lane S) compared to the His-control protein. T: Total Lysate, S: Cleared Soluble Fraction, I: Insoluble Pellet.

Material available for download
Expresso Solubility and Expression Screening System user manual
Expresso Solubility and Expression Screening System SDS
Expresso Solubility and Expression Screening System applicationnote
Expresso Solubility and Expression Screening System poster
Expresso Solubility and Expression Screening System flyer

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