The Expresso® Rhamnose cloning and protein expression systems from Lucigen are designed for fast, easy and efficient directional cloning and expression of PCR-amplified genes using Expressioneering™ Technology. Expressioneering Technology uses in vivo homologous recombination to seamlessly clone PCR amplified DNA into specially designed expression vectors without the need for enzymes or purification steps. A single host strain is used for both stable cloning and controlled protein expression, making Expresso Rhamnose the fastest cloning and expression systems available. Systems are supplied complete with pre-processed expression plasmids and competent cells, supplied in single transformation vials.
Benefits of using the Expresso rhamnose cloning and protein expression system
• Five-second, directional, enzyme-free PCR cloning
• Single competent cell host strain for both cloning and expression
• Tigher expression control with tunable Rhamnose (rhaPBAD)
• High throughput format friendly with autoinduction reagents
• Available with cleavable SUMO solubility tag
Expressioneering technology uses in vivo homologous recombination to seamlessly clone PCR amplified DNA into specially designed expession vectors without the need for enzymes or purification steps.
Material available for download
Expresso Rhamnose cloning and expression system manual
Expresso Rhamnose SUMO cloning and expression system manual
Nature application note: Expresso cloning and expression sysems: Expressioneering technology streamlines recombinant protein expression
Poster: Expresso cloning and expression systems