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Exosome isolation and purification: ExoQuick

The ExoQuick® portfolio of products from System Biosciences simplifies exosome isolation with high-throughput, quantitative isolation of exosomes from low sample volumes. Compatible with any biofluid and a wide variety of downstream applications, the ExoQuick range is an effective and proven alternative to ultracentrifugation. 

Benefits of using ExoQuick
• Compatible with any biofluid
• Low sample input requirements 
• No ultracentrifugation 
• High yield of functional, high quality exosomes

Choose the right ExoQuick for your application 

Application
Product
Purest EV isolation
ExoQuick ULTRA
ExoQuick-TC ULTRA
General purpose EV isolation
ExoQuick
ExoQuick-TC
EV isolation for pre-clinical/in vivo studies
ExoQuick-CG
EV isolation that removes contaminating lipoprotein particles from plasma or serum
ExoQuick-LP
EV isolation that includes a de-fibrinating plasma step prior to isolation
ExoQuick Plasma Prep with Thrombin

 

ExoQuick delivers high protein concentrations

Exosomes were isolated from ovarian tumor ascites fluid using ExoQuick, chromatography, DynaBeads, or ultracentrifugation. The ExoQuick method consistently delivered higher concentrations of protein than the other three isolation methods used

Exosomes were isolated from ovarian tumor ascites fluid using ExoQuick, chromatography, DynaBeads, or ultracentrifugation. The ExoQuick method consistently delivered higher concentrations of protein than the other three isolation methods used.

ExoQuick ULTRA delivers high yields of clean exosomes

ExoQuick ULTRA delivers high yields of clean exosomes

(A) A coomassie blue-stained protein gel comparing the protein content of exosome preps isolated using different methods shows only a few, defined protein bands in the ExoQuick ULTRA lane compared to the other methods. (B) Western blotting of the gel shows that the ExoQuick ULTRA prep contains the highest levels of exosome-specific markers CD9, CD81, and Hsp70 and the lowest levels of the carryover proteins albumin and IgGH. In contrast, the prep from Company Q appears to be primarily albumin, and even the sample prepared using ultracentrifugation contains considerably higher levels of both albumin and IgGH. Each lane was loaded with 7 μg of total protein as measured using a fluorometric Qubit protein assay

SeraMir™ RNA Purification
We also offer an all-in-one exosome RNA isolation and purification kit. Combining ExoQuick and SeraMir RNA columns for the purification of exosomal RNA, the SeraMir kit enables researchers to go from sample to amplified exoRNAs in one day. Find out more

Applications
• Biomarker studies 
• Exosomal miRNA profiling 
• Exosomal proteomics
• Exosomal lipidomics/metabolomics
• Functional studies, such as cell-to-cell signalling 

Material available for download
ExoQuick ULTRA User Manual
ExoQuick-TC ULTRA User Manual
ExoQuick user manual
ExoQuick-TC datasheet
ExoQuick-TC user manual
ExoQuick-CG user manual
ExoQuick-LP user manual
Protocol: Thrombin plasma prep for exosome precipitation

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
ExoQuick exosome precipitation solution (5 ml) EXOQ5A-1 · System Biosciences
EXOQ5A-1
System Biosciences
5 ml £312.00
5 ml
view
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL) EXOQ5TM-1 · System Biosciences
EXOQ5TM-1
System Biosciences
75 reactions £707.00
75 reactions
view
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL) TMEXO-1 · System Biosciences
TMEXO-1
System Biosciences
100 reactions £476.00
100 reactions
view
ExoQuick exosome precipitation solution (20 ml) EXOQ20A-1 · System Biosciences
EXOQ20A-1
System Biosciences
20 ml £1006.00
20 ml
view
ExoQuick TC 10ml EXOTC10A-1 · System Biosciences
EXOTC10A-1
System Biosciences
10 ml £311.00
10 ml
view
ExoQuick TC 50ml EXOTC50A-1 · System Biosciences
EXOTC50A-1
System Biosciences
50 ml £1044.00
50 ml
view
ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml) EXOCG50A-1 · System Biosciences
EXOCG50A-1
System Biosciences
50 ml £1007.00
50 ml
view
Exosome-depleted FBS Media Supplement EXO-FBS-50A-1 · System Biosciences
EXO-FBS-50A-1
System Biosciences
50 ml £171.00
50 ml
view
Exosome-depleted FBS Media Supplement EXO-FBS-250A-1 · System Biosciences
EXO-FBS-250A-1
System Biosciences
250 ml £741.00
250 ml
view
ExoQuick-LP for lipoprotein pre-clear & exosome isolation EXOLP5A-1 · System Biosciences
EXOLP5A-1
System Biosciences
5 reactions £431.00
5 reactions
view
ExoQuick ULTRA EV Isolation Kit for Serum and Plasma EQULTRA-20A-1 · System Biosciences
EQULTRA-20A-1
System Biosciences
20 reactions £472.00
20 reactions
view
ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media EQULTRA-20TC-1 · System Biosciences
EQULTRA-20TC-1
System Biosciences
20 reactions £472.00
20 reactions
view