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Exosome RNA amplification & profiling: SeraMir

The SeraMir™ kit from System Biosciences enables researchers to go from serum sample to amplified exosomal RNAs in just one day. Using ExoQuick™ solution to isolate exosomes, the SeraMir kit enables the 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during cDNA synthesis ready for qPCR. Primers for PCR amplification are included to make double-stranded cDNA that is ready to generate sense-strand exoRNAs for T7 IVT, with amplified exoRNAs suitable for microarrays and next-generation sequencing applications.

Benefits of using SeraMir
• Fast and simple one-step exosome isolation - no ultracentrifugation
• Cross species and sample compatibility
• Low sample requirements
• Compatible with frozen sample types
• Produces cleaner RNA - ideal for downstream applications
• Reduces variability - isolate exosomes first
• Increased sensitivity - amplified exoRNAs
• Compatible with qPCR

How SeraMir works

Isolate serum exosomes and purify exoRNAs

SeraMir: Isolate serum exosomes and purify exoRNAs

Tail exoRNAs and synthesise double-tagged cDNA

SeraMir: Tail exoRNAs and synthesize double-tagged cDNA

SeraMir

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

SeraMir: Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.

Material available for download
AACR SeraMir poster
SeraMir Exosome RNA Amplification Kit product sheet
SeraMir Exosome RNA Amplification Kit user manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
SeraMir Exosome RNA Ampflication Kit RA800A-1 · System Biosciences
RA800A-1
System Biosciences
20 reactions £770.00
20 reactions
view
SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns) RA800TC-1 · System Biosciences
RA800TC-1
System Biosciences
10 reactions £542.00
10 reactions
view
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns) RA806A-1 · System Biosciences
RA806A-1
System Biosciences
20 preparations £450.00
20 preparations
view
SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps RA806TC-1 · System Biosciences
RA806TC-1
System Biosciences
10 preparations £450.00
10 preparations
view
SeraMir Exosome RNA Purification Column kit (20 exoRNA columns) RA808A-1 · System Biosciences
RA808A-1
System Biosciences
20 preparations £314.00
20 preparations
view
SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set RA810A-1 · System Biosciences
RA810A-1
System Biosciences
20 profiles £427.00
20 profiles
view
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components) RA820A-1 · System Biosciences
RA820A-1
System Biosciences
20 profiles £1111.00
20 profiles
view
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components) RA820TC-1 · System Biosciences
RA820TC-1
System Biosciences
20 profiles £1396.00
20 profiles
view
Mouse SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set (20 assays) RA811A-1 · System Biosciences
RA811A-1
System Biosciences
20 profiles £427.00
20 profiles
view
Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA811A-1 components) 20 profiles RA821A-1 · System Biosciences
RA821A-1
System Biosciences
20 profiles £1111.00
20 profiles
view
Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components) RA821TC-1 · System Biosciences
RA821TC-1
System Biosciences
20 profiles £1396.00
20 profiles
view
Rat SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set RA812A-1 · System Biosciences
RA812A-1
System Biosciences
20 profiles £427.00
20 profiles
view
Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA812A-1 components) RA822A-1 · System Biosciences
RA822A-1
System Biosciences
20 profiles £1111.00
20 profiles
view
Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components) RA822TC-1 · System Biosciences
RA822TC-1
System Biosciences
20 profiles £1396.00
20 profiles
view
SeraMir Control spike-in Small RNA and qPCR assay RA805A-1 · System Biosciences
RA805A-1
System Biosciences
100 assays £166.00
100 assays
view
SeraMir 3'-Universal Primer (10,000 qPCR reactions) RA800P-3 · System Biosciences
RA800P-3
System Biosciences
10000 reactions £115.00
10000 reactions
view