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Selective exosome capture: Exo-Flow

The Exo-Flow™ magnetic antibody bead kit from System Biosciences enables the selective capture and purification of exosomes based on specific surface markers. Available in two different formats, the Exo-Flow 32 and 96 IP kits can be used for high-throughput, selective exosome purification in 32 well or 96 well plate formats, whilst the Exo-Flow kits for FACS enables flow sorting to quantify and purify distinct subpopulations of exosomes. The 9.1 um diameter and high binding capacity of the Exo-Flowmagnetic beads enable researchers to capture billions of exosomes in a single binding reaction. 

The second-generation Exo-flow 2.0 kits are available for popular tetraspanin markers including CD63, CD9, and CD81, different versions available for source of EVs serum/plasma or tissue culture media for undetectable background binding. 

Benefits of using the Exo-Flow Kits for IP and FACS 
• Selectively capture billions of exosomes
• Capture rare exosomes present in very low numbers
• Enhanced immunomagnetic beads
• Multiple capture antibodies to choose from
• Modular system to customise captures
• Reversible Exo-FITC flow stain for flow sorting

Structure of Exo-Flow beads

Exo-Flow bead structure

Exo-flow 2.0 beads have an outer silica coating which delivers undetectable background binding.

CD63 Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolation

Bead flow separation data for exosomes secreted by HEK293 cells and captured using the CD63 Exo-Flow Capture Kit.

Exo-Flow 2.0 delivers undetectable background binding when analysed using flow cytometry

Exo-Flow 2.0 delivers undetectable background binding when analysed using flow cytometry.

Material available for download
Exo-Flow & Exo-Flow IP user manual
Exo-Flow product sheet
Exo-Flow 2.0 for serum or plasma manual
Exo-Flow 2.0 for tissue culture media manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CD9 Exo-Flow capture kit (Magnetic streptavidin beads, CD9-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW100A-1 · System Biosciences
EXOFLOW100A-1
System Biosciences
10 reactions £541.00
10 reactions
view
Tetraspanin Exo-Flow Combo capture kit (Magnetic streptavidin beads, CD9, CD63, and CD81-biotin capture antibodies, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW150A-1 · System Biosciences
EXOFLOW150A-1
System Biosciences
10 reactions £821.00
10 reactions
view
CD31 Exo-Flow capture kit (Magnetic streptavidin beads, CD31-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW200A-1 · System Biosciences
EXOFLOW200A-1
System Biosciences
10 reactions £541.00
10 reactions
view
CD44 Exo-Flow capture kit (Magnetic streptavidin beads, CD44-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW210A-1 · System Biosciences
EXOFLOW210A-1
System Biosciences
10 reactions £541.00
10 reactions
view
CD63 Exo-Flow capture kit (Magnetic streptavidin beads, CD63-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW300A-1 · System Biosciences
EXOFLOW300A-1
System Biosciences
10 reactions £541.00
10 reactions
view
CD81 Exo-Flow capture kit (Magnetic streptavidin beads, CD81-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW400A-1 · System Biosciences
EXOFLOW400A-1
System Biosciences
10 reactions £541.00
10 reactions
view
Rab5b Exo-Flow capture kit (Magnetic streptavidin beads, Rab5b-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW500A-1 · System Biosciences
EXOFLOW500A-1
System Biosciences
10 reactions £541.00
10 reactions
view
HLA-G Exo-Flow capture kit (Magnetic streptavidin beads, HLA-G-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW600A-1 · System Biosciences
EXOFLOW600A-1
System Biosciences
10 reactions £541.00
10 reactions
view
CD14 Exo-Flow capture kit (Magnetic streptavidin beads, CD14-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW610A-1 · System Biosciences
EXOFLOW610A-1
System Biosciences
10 reactions £541.00
10 reactions
view
CD68 Exo-Flow capture kit (Magnetic streptavidin beads, CD68-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW620A-1 · System Biosciences
EXOFLOW620A-1
System Biosciences
10 reactions £541.00
10 reactions
view
EpCAM Exo-Flow capture kit (Magnetic streptavidin beads, EpCAM-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) EXOFLOW630A-1 · System Biosciences
EXOFLOW630A-1
System Biosciences
10 reactions £541.00
10 reactions
view
Exo-Flow Multifunctional Magnetic Stand EXOFLOW700A-1 · System Biosciences
EXOFLOW700A-1
System Biosciences
1 kit £439.00
1 kit
view
Exo-FITC Exosome FACS stain EXOFLOW800A-1 · System Biosciences
EXOFLOW800A-1
System Biosciences
20 reactions £204.00
20 reactions
view
Exo-APC Exosome FACS stain EXOFLOW810A-1 · System Biosciences
EXOFLOW810A-1
System Biosciences
20 reactions £204.00
20 reactions
view
Basic Exo-Flow kit without any coupled antibodies (Uncoupled magnetic streptavidin beads, Wash and Elution Buffers, Exo-FITC stain) CSFLOWBASICA-1 · System Biosciences
CSFLOWBASICA-1
System Biosciences
100 reactions £922.00
100 reactions
view