Selective exosome capture: Exo-Flow

The Exo-Flow™ magnetic antibody bead kit from System Biosciences enables the selective capture and purification of exosomes based on specific surface markers. Available in two different formats, the Exo-Flow 32 and 96 IP kits can be used for high-throughput, selective exosome purification in 32 well or 96 well plate formats, whilst the Exo-Flow kits for FACS enables flow sorting to quantify and purify distinct subpopulations of exosomes. The 9.1 um diameter and high binding capacity of the Exo-Flowmagnetic beads enable researchers to capture billions of exosomes in a single binding reaction. 

The second-generation Exo-flow 2.0 kits are available for popular tetraspanin markers including CD63, CD9, and CD81, different versions available for source of EVs serum/plasma or tissue culture media for undetectable background binding. 

Benefits of using the Exo-Flow Kits for IP and FACS 
• Selectively capture billions of exosomes
• Capture rare exosomes present in very low numbers
• Enhanced immunomagnetic beads
• Multiple capture antibodies to choose from
• Modular system to customise captures
• Reversible Exo-FITC flow stain for flow sorting

Structure of Exo-Flow beads

Exo-flow 2.0 beads have an outer silica coating which delivers undetectable background binding.

CD63 Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolation

Exo-Flow 2.0 delivers undetectable background binding when analysed using flow cytometry.

Material available for download
Exo-Flow & Exo-Flow IP user manual
Exo-Flow product sheet
Exo-Flow 2.0 for serum or plasma manual
Exo-Flow 2.0 for tissue culture media manual