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Exceptional yield exosome purification kit: Exo-spin

The Exo-spin™ exosome purification kit from Cell Guidance Systems purifies functional, whole exosomes (30-400 nm size) with superior yields and higher quality than many other alternative technologies. Based on a unique combination of precipitation and size exclusion chromatography purification with no organic buffers and requiring no ultracentrifugation, the Exo-spin kits are compatible with a variety of biological fluids including cell culture media, urine and saliva. The Exo-spin blood kits are suitable for use with blood, plasma and serum.

Benefits of using the Exo-spin kits
• Isolate intact whole exosomes
• Excellent yield and high levels of purity
• No ultracentrifugation required
• Simple and reliable
• Ultra-low protein and rRNA contamination
• No organic buffers
• Fast one hour protocol
• Wide sample compatibility

Cell culture medium, saliva, urine, human breast milk*, cerebrospinal fluid*
& any low protein biological fluids (1 x 109 particles/ml)
Sample starting volume
(per Exo-spin column)
Isolation method
Exo-spin kit
<1 ml to 50 ml Precipitation + SEC (centrifugation)
EX01
<1 ml to 75 ml Precipitation + SEC (fractionation by gravity flow)
EX05
75 ml to 500 ml Precipitation + SEC (fractionation by gravity flow)
EX04
Blood samples (plasma and sera)†
SERA
≤ 100 µl SEC (centrifugation) EX03
≤ 150 µl SEC (fractionation by gravity flow) EX05
100 µl to 500 µl Precipitation + SEC (centrifugation) EX02
1 ml SEC (fractionation by gravity flow) EX04
PLASMA
≤ 150 µl SEC (fractionation by gravity flow) EX05
100 µl to 250 µl Precipitation + SEC (centrifugation) EX02
1 ml SEC (fractionation by gravity flow) EX04

* For cerebrospinal fluid (CSF) and human breast milk samples, validated protocols are available for EX01 only. The protocols are provided in the user guides.
† Highly concentrated exosome samples (e.g. 1x1012 particles/ml) other than blood can also be used.

EX02 Exo-spin kit comparative data

EX02 Exo-spin™ kit comparative data

EX02 Exo-spin™ kit comparative data

Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method and biofluid triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018).

EX04 Exo-spin midi columns comparative data

EX04 Exo-spin™ midi columns comparative data

Comparison of total exosome recovery from 10 ml conditioned cell culture medium. The total particle yield obtained with Exo-spin buffer + Exo-spin midi columns is greater than the yield obtained using the membrane affinity method. Notably, Exo-spin™ buffer is much more efficient than generic PEG 6000 when used prior to Exo-spin midi column exosome isolation.

Downstream applications
• RNA studies
• Mass spectrometry
• Western blotting/ELISA
• Nano particle tracking and functional studies

Exo-spin citations

Material available for download
Exo-spin user guide
Application note: Exosome isolation from CSF
Application note: Comparison between precipitation & size exclusion chromatography (SEC) vs. membrane affinity for exosome isolation

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Exo-spin columns EX03-8 · Cell Guidance Systems
EX03-8
Cell Guidance Systems
8 columns £67.00
8 columns
view
Exo-spin columns EX03-25 · Cell Guidance Systems
EX03-25
Cell Guidance Systems
24 columns £173.00
24 columns
view
Exo-spin blood, 8 columns EX02-8 · Cell Guidance Systems
EX02-8
Cell Guidance Systems
8 columns £112.00
8 columns
view
Exo-spin blood, 24 columns EX02-25 · Cell Guidance Systems
EX02-25
Cell Guidance Systems
24 columns £312.00
24 columns
view
Exo-spin blood, 48 columns EX02-50 · Cell Guidance Systems
EX02-50
Cell Guidance Systems
48 columns £550.00
48 columns
view
Exo-spin midi columns EX04-5 · Cell Guidance Systems
EX04-5
Cell Guidance Systems
5 columns £139.00
5 columns
view
Exo-spin midi columns EX04-20 · Cell Guidance Systems
EX04-20
Cell Guidance Systems
20 columns £395.00
20 columns
view
Exo-spin columns EX03-50 · Cell Guidance Systems
EX03-50
Cell Guidance Systems
48 columns £273.00
48 columns
view
Exo-spin mini-HD columns EX05-100 · Cell Guidance Systems
EX05-100
Cell Guidance Systems
100 columns £1612.00
100 columns
view
Exo-spin mini-HD columns EX05-5 · Cell Guidance Systems
EX05-5
Cell Guidance Systems
5 columns £89.00
5 columns
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Exo-spin mini-HD columns EX05-20 · Cell Guidance Systems
EX05-20
Cell Guidance Systems
20 columns £345.00
20 columns
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Exo-spin standard, 8 columns, 60 ml buffer EX01-8 · Cell Guidance Systems
EX01-8
Cell Guidance Systems
8 columns £117.00
8 columns
view
Exo-spin standard, 24 columns, 250 ml buffer EX01-25 · Cell Guidance Systems
EX01-25
Cell Guidance Systems
24 columns £334.00
24 columns
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Exo-spin standard, 24 columns, 500 ml buffer EX01-25L · Cell Guidance Systems
EX01-25L
Cell Guidance Systems
24 columns £395.00
24 columns
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Exo-spin standard, 48 columns, 500 ml buffer EX01-50 · Cell Guidance Systems
EX01-50
Cell Guidance Systems
48 columns £600.00
48 columns
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Exo-spin Buffer EX06-250 · Cell Guidance Systems
EX06-250
Cell Guidance Systems
250 ml £178.00
250 ml
view
Exo-spin Buffer EX06-30 · Cell Guidance Systems
EX06-30
Cell Guidance Systems
30 ml £39.00
30 ml
view