The Exo-spin™ exosome purification kit from Cell Guidance Systems purifies functional, whole exosomes (30-400 nm size) with superior yields and higher quality than many other alternative technologies. Based on a unique combination of precipitation and size exclusion chromatography purification with no organic buffers and requiring no ultracentrifugation, the Exo-spin kits are compatible with a variety of biological fluids including cell culture media, urine and saliva. The Exo-spin blood kits are suitable for use with blood, plasma and serum.
Benefits of using the Exo-spin kits
• Isolate intact whole exosomes
• Excellent yield and high levels of purity
• No ultracentrifugation required
• Simple and reliable
• Ultra-low protein and rRNA contamination
• No organic buffers
• Fast one hour protocol
• Wide sample compatibility
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Cell culture medium, saliva, urine, human breast milk*, cerebrospinal fluid*
& any low protein biological fluids (1 x 109 particles/ml)
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Sample starting volume
(per Exo-spin column)
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Isolation method
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Exo-spin kit
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| <1 ml to 50 ml | Precipitation + SEC (centrifugation) |
EX01
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| <1 ml to 75 ml | Precipitation + SEC (fractionation by gravity flow) |
EX05
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| 75 ml to 500 ml | Precipitation + SEC (fractionation by gravity flow) |
EX04
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Blood samples (plasma and sera)†
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Sera
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| < 100 µl | SEC (centrifugation) | EX03 |
| < 150 µl | SEC (fractionation by gravity flow) | EX05 |
| 100 µl to 500 µl | Precipitation + SEC (centrifugation) | EX02 |
| 1 ml | SEC (fractionation by gravity flow) | EX04 |
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Plasma
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| < 150 µl | SEC (fractionation by gravity flow) | EX05 |
| 100 µl to 250 µl | Precipitation + SEC (centrifugation) | EX02 |
| 1 ml | SEC (fractionation by gravity flow) | EX04 |
* For cerebrospinal fluid (CSF) and human breast milk samples, validated protocols are available for EX01 only. The protocols are provided in the user guides.
† Highly concentrated exosome samples (e.g. 1x1012 particles/ml) other than blood can also be used.
EX02 Exo-spin kit comparative data
Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method and biofluid triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018).
EX04 Exo-spin midi columns comparative data
Comparison of total exosome recovery from 10 ml conditioned cell culture medium. The total particle yield obtained with Exo-spin buffer + Exo-spin midi columns is greater than the yield obtained using the membrane affinity method. Notably, Exo-spin™ buffer is much more efficient than generic PEG 6000 when used prior to Exo-spin midi column exosome isolation.
Downstream applications
• RNA studies
• Mass spectrometry
• Western blotting/ELISA
• Nano particle tracking and functional studies
Material available for download
Exo-spin user guide: cell culture media, urine, saliva
Exo-spin user guide: blood sera & plasma
Exo-spin mini columns user guide: blood sera, cell culture media & labelled exosome samples
Exo-spin midi columns user guide: cell culture media, saliva, urine, blood plasma & sera
Exo-spin mini HD columns user guide: cell culture media, saliva, urine, blood plasma & sera
Application note: exosome isolation from CSF
Application note: comparison between precipitation & size exclusion chromatography (SEC) vs. membrane affinity for exosome isolation
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| Exo-spin Buffer, 250 ml EX06-250 · Cell Guidance Systems | EX06-250 Cell Guidance Systems |
250 ml |
£204.00
250 ml
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| Exo-spin Buffer, 30 ml EX06-30 · Cell Guidance Systems | EX06-30 Cell Guidance Systems |
30 ml |
£49.00
30 ml
view
|
