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Ultimate Western Blot Imaging System - Azure 600
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Radiance chemiluminescence Western blot substrates

Radiance chemiluminescence Western blot substrates

Azure Biosystems' highly stable and bright chemiluminescent Radiance substrates are for use in Western blotting. Providing the largest dynamic range of any chemiluminescent substrate, Radiance ECL is not depleted at high protein loads giving improved quantitative accuracy. The Radiance Plus format has attomole sensitivity which together with the long-lasting signal, enable the detection of bands not visualised with other substrates.

Radiance substrate comparison

Substrate
Features
Radiance ECL
Economical 
 
Linear signal for quantitative Westerns
Radiance Plus
Ultra-high sensitivity for low abundance proteins
Radiance Q
Higher linear dynamic range for more reliable quantitation
 
Increased sensitivity

Extended signal longevity

Radiance serial dilution

A serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the Radiance Substrate indicated.

Radiance substrates are better optimised for quantitation than alternatives

Chemiluminescence Comparison With Radiance Substrates

Radiance ECL shows significantly less signal decay than other substrates

The blots pictured to the right were imaged  at regular intervals up to sixty minutes, and  the signal of the second spot is plotted above. Radiance ECL shows significantly less signal  decay than other substrates

The blots pictured to the right were imaged at regular intervals up to sixty minutes, and the signal of the second spot is plotted above.Radiance ECL shows significantly less signal decay than other substrates.

Radiance ECL signal linearity

linearity of the Radiance ECL

 

Radiance ECL Signal Linearity

 

 

 

 

 

 

 

 

 

 

A 1:1 serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the substrate indicated.The linearity of the Radiance ECL signal is shown on the bottom.

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