Radiance chemiluminescence Western blot substrates

The Radiance series of HRP substrates for chemiluminescent Western blots from Azure Biosystems generate long lasting signals and extreme sensitivity. 

Radiance ECL
Radiance ECL is an HRP substrate with long lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other ECL substrates.

Radiance Plus 
Radiance Plus is the most sensitive HRP substrate for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualised with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands. 

Radiance Q
Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low-abundance proteins. As the substrate is not depleted at high protein loads, Radiance Q provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

Radiance substrate comparison

Substrate
Features
Radiance ECL
Economical 
Linear signal for quantitative Westerns
Radiance Plus
Ultra-high sensitivity for low abundance proteins
Radiance Q
Higher linear dynamic range for more reliable quantitation
Increased sensitivity
Extended signal longevity

 

Radiance serial dilution

A serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the Radiance Substrate indicated.

Radiance substrates are better optimised for quantitation than alternatives

Chemiluminescence Comparison With Radiance Substrates

Radiance ECL shows significantly less signal decay than other substrates

The blots pictured to the right were imaged  at regular intervals up to sixty minutes, and  the signal of the second spot is plotted above. Radiance ECL shows significantly less signal  decay than other substrates

The blots pictured to the right were imaged at regular intervals up to sixty minutes, and the signal of the second spot is plotted above. Radiance ECL shows significantly less signal decay than other substrates.

Radiance ECL signal linearity

Radiance ECL signal linearity

A 1:1 serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the substrate indicated.The linearity of the Radiance ECL signal is shown on the bottom.

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Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Radiance Q AC2101 · Azure Biosystems
AC2101
Azure Biosystems
150 ml £250.00
150 ml
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Radiance ECL AC2204 · Azure Biosystems
AC2204
Azure Biosystems
500 ml £200.00
500 ml
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Radiance Plus AC2103 · Azure Biosystems
AC2103
Azure Biosystems
150 ml £290.00
150 ml
view