Watch: why does sensitivity matter for HTS assays?

Assay sensitivity is a critical factor in high-throughput screening (HTS), enabling lower enzyme usage and greater experimental flexibility. Transcreener assays are up to ten times more sensitive than alternative methods, reducing assay costs by allowing significantly less enzyme to be used. Lower enzyme concentrations also improve the accurate measurement of inhibitor potency, helping researchers more effectively differentiate and rank compounds. In addition, Transcreener's high sensitivity supports assays under initial velocity conditions with substrate concentrations at or below the Km value, making it well suited for identifying competitive inhibitors, even when working with slow enzymes.