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RNA & In Vitro Transcription Solutions
APExBIO offers a comprehensive portfolio of RNA research products and in vitro transcription solutions designed to support the development of mRNA therapeutics, vaccines, gene editing technologies and functional genomics studies. From high-yield RNA synthesis enzymes and modified nucleotides to purification reagents and custom RNA production services, APExBIO provides researchers with the tools needed to streamline RNA workflows from design through to application.
Whether you are generating mRNA for protein expression studies, producing sgRNA for CRISPR experiments, or developing next-generation RNA therapeutics, APExBIO delivers reliable reagents, scalable manufacturing capabilities and high-quality RNA products to support your research.
Key Features
APExBIO supports every stage of the RNA production process from DNA template preparation to custom RNA synthesis:
- High-yield T7, SP6 and T3 in vitro transcription systems
- Modified nucleotides for enhanced mRNA stability and translation
- Complete workflow from transcription to RNA purification
- Custom mRNA, circular RNA and self-amplifying RNA (saRNA) synthesis services
- Solutions for mRNA therapeutics, vaccines and CRISPR research
RNA products & Services
Catalogue RNA – Premade mRNA, circRNA & saRNA
Researchers seeking to accelerate their studies can choose from an extensive catalogue of pre-made RNA products, including a comprehensive catalogue of premade RNA products, including mRNA, circRNA, and saRNA. All are incorporated with EZ Cap (Cap1) or ARCA (Cap0), polyA Tail and Modified Nucleotides（e.g. pseudo-UTP, 5-moUTP) and validated to ensure high integrity, potency in performance in a variety of applications.
| Type | Linear RNA | Circular RNA | Self-amplifying RNA |
|---|---|---|---|
| Structure | Linear. 5' and 3' ends | Closed circle | Linear. 5' and 3' ends |
| Function of Cap1/Cap0 |
Protect from degradation. Facilitate nuclear export and splicing |
No need of Cap |
Protect from degradation. Facilitate nuclear export and splicing |
| Function of PolyA Tail |
Enhance RNA stability Facilitate translation |
No need of polyA tail |
Enhance RNA stability Facilitate translation |
| Function of Modified Nucleotides |
Reduce immunogenicity Facilitate translation |
No need of modified nucleotides |
Reduce immunogenicity Facilitate translation |
| Ribosome Recruitment | Need 5'Cap for ribosome recruitment and translation initiation | Need Internal Ribosome Entry Site (IRES) for translation initiation | Need 5'Cap for ribosome recruitment and translation initiation |
| Protein Expression | High peak, transient | Lower peak, more sustained | Very high, sustained (low dose effective) |
| Half Life | Short (hours) | Long (days) | Functionally long (via replication) |
| Immunogenicity | Moderate -> high (unless modified) | Low (reduced innate sensing) | High (dsRNA intermediates trigger sensors) |
Product Types:
► Reporter Gene mRNA ► Self-Amplifying RNA
► Tumour-associated mRNA ► Cicular RNA
► Interleukin mRNA ► Organelle labeled mRNA
► Genome Editing mRNA ► Other Catalog mRNA
In vitro Transcription
HyperScribe™ In Vitro Transcription Kits provide rapid, high-yield synthesis of high-quality RNA for research applications. Optimised for performance and reproducibility, these kits support the production of mRNA, sgRNA and labelled RNA using T7, SP6 or T3 RNA polymerase systems. These kits are designed to simplify workflow implementation whilst maintaining excellent transcript integrity and yield.
Modified Nucleotides
We offer a broad range of modified nucleotides for in vitro transcription applications, enabling researchers to improve RNA stability, enhance translation efficiency and reduce innate immune responses. The portfolio includes commonly used modifications such as pseudo-UTP and N1-methyl-pseudo-UTP, together with fluorescently labelled and biotin-labelled nucleotides for RNA tracking, imaging and detection application
Custom RNA Synthesis Services
Custom RNA synthesis services are available in microgram to gram scale to support a wide range of research applications, from early-stage discovery to therapeutic development. Researchers can choose from linear mRNA, circRNA and saRNA formats, with options for sequence optimisation, nucleotide modification, capping and poly(A) tailing and can be combined with downstream LNP encapsulation for a complete RNA production workflow.
Service Options:
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Linear RNA Synthesis Capping and Modified Nucleotides Sequence Optimization Microgram to Gram Scale |
Circular RNA Synthesis High Circularization Efficiency 100 bp to 5000 bp Gene Insert High and Sustained Expression Level |
Self-Amplifying RNA Synthesis Optimized Vector and Replicase Capping and Modified Nucleotides Less for Higher Expression Level |
LNP Encapsulation High Encapsulation Efficiency Compatible with mRNA/siRNA/plasmid Dry Ice Shipping |
GMP Grade Materials
We supply GMP-grade raw materials to support mRNA and RNA therapeutic development and manufacturing. All products are manufactured under GMP-compliant quality systems with stringent controls to ensure consistency, purity, bioactivity and low contamination levels.
Available GMP-Grade Products
► EZ Cap™ Reagent AG
► Pseudo-UTP
► T7 RNA Polymerase
► Inorganic Pyrophosphatase (E. coli)
► RNase-free DNase I
► RNase-free BsaI and supporting enzymes