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CytoSelect™ Cell Viability and Cytotoxicity Assay

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96 assays _$$_
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£445.00 Estimated delivery Wed 24 Jul
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The CytoSelect™ Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

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Storage Temperature:

Store the Calcein AM and Ethidium Homodimer at -20°C. Store all remaining kit components at 4°C.

Viability of Human Foreskin Fibroblasts.
Viability of Human Foreskin Fibroblasts. BJ-TERT cells were seeded at 50,000 cells/well and allowed to culture for 24 hours. Cells were then treated with and without Saponin. All cells were then stained with Calcein AM or EthD-1. A: Cells without Saponin treatment stained with Calcein AM. B: Cells without Saponin treatment stained with EthD-1. C: Cells with Saponin treatment stained with Calcein AM. D: Cells with Saponin treatment stained with EthD-1.
Viability of Human Foreskin Fibroblasts.
Viability of Human Foreskin Fibroblasts. BJ-TERT cells were seeded at 50,000 cells/well and allowed to culture for 24 hours. Cells were then treated with and without Saponin. All cells were then stained with Calcein AM or EthD-1. A: Cells without Saponin treatment stained with Calcein AM. B: Cells without Saponin treatment stained with EthD-1. C: Cells with Saponin treatment stained with Calcein AM. D: Cells with Saponin treatment stained with EthD-1.
Viability of Human Foreskin Fibroblasts.
Viability of Human Foreskin Fibroblasts. BJ-TERT cells were seeded at 50,000 cells/well and allowed to culture for 24 hours. Cells were then treated with and without Saponin. All cells were then stained with Calcein AM or EthD-1. A: Cells without Saponin treatment stained with Calcein AM. B: Cells without Saponin treatment stained with EthD-1. C: Cells with Saponin treatment stained with Calcein AM. D: Cells with Saponin treatment stained with EthD-1.
Viability of Human Foreskin Fibroblasts.
Viability of Human Foreskin Fibroblasts. BJ-TERT cells were seeded at 50,000 cells/well and allowed to culture for 24 hours. Cells were then treated with and without Saponin. All cells were then stained with Calcein AM or EthD-1. A: Cells without Saponin treatment stained with Calcein AM. B: Cells without Saponin treatment stained with EthD-1. C: Cells with Saponin treatment stained with Calcein AM. D: Cells with Saponin treatment stained with EthD-1.
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