Cancer cell transmigration, particularly extravasation, is an important step in cancer metastasis. It is the final step in a cascade of interactions between cells and the endothelium. CytoSelect™ Leukocyte Transmigration Assay provides a robust system for the quantitative determination of transmigrations and interactions between endothelium and leukocytes. Migratory cells may be quantified on a fluorescence plate reader. Kits use 24-well plates with 3 µm pore size membrane inserts.
Biochemical and cellular assays
LeukoTracker™ Solution and TNFa should be removed from the kit and stored at -20°C immediately. Stor