Hydrogen Peroxide Assay Kit

Cayman’s Hydrogen Peroxide Assay Kit provides fluorometric or colorimetric methods for the quantification of extracellular H2O2 produced by cultured cells. In the presence of horseradish peroxidase (HRP), H2O2 reacts stoichiometrically with an HRP substrate, ADHP, to produce the compound resorufin.{33076,13685} Resorufin fluorescence can be easily quantified at excitation and emission wavelengths of 530-540 and 585-595 nm, respectively. Alternatively, the absorbance of resorufin can be measured at 570 nm. Catalase, an H2O2 scavenger, is included in the kit for verifying the specificity of the assay. ROS, such as H2O2 and superoxide, are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.{29448} This kit provides a valuable method for immunologists to assess the capacity of immune cell killing using ROS. This assay offers the option to measure absorbance or fluorescence. It is at the user’s discretion to choose the mode of measurement (and corresponding standard curve preparation) that best fits their needs. When read fluorometrically, this assay has a range of 0-10 µM and a lower limit of detection (LLOD) of 0.02 µM. When read colorimetrically, the range is 0-80 µM with an LLOD of 0.15 µM.