H. sapiens (Human)
CD32 antigen, CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIc receptor, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RII
CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body. Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 3000pg/ml, 1500pg/ml, 750pg/ml, 419.pg/ml, 187.5pg/ml, 93.8pg/ml, 46.9pg/ml human CD32 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well) . Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. We recommend that each human CD32 standard solution and each sample is measured in duplicate.
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at ro