TBARS Assay Kit

Cayman’s TBARS Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and whole cell lysates. The MDA-TBA adduct formed by the reaction of MDA and TBA under high temperature (90-100°C) and acidic conditions is measured colorimetrically at 530-540 nm or fluorometrically at an excitation wavelength of 530 nm and an emission wavelength of 550 nm. Although this reaction has a much higher sensitivity when measured fluorometrically, protocols for both methods are provided. When read fluorometrically, this assay has a range of 0.0625-5 µM of MDA equivalents and when read colorimetrically, the assay range is 0.625-50 µM of MDA equivalents.