Hoechst 33342

• Data sheet: View or download
• MSDS: View or download

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Either dye can be used to stain live or fixed cells in buffer or medium at 1 ug/mL with no wash step. Biotium also offers Hoechst 33342, 10 mg/mL in H2O. 

 
• &lamba;_Ex/&lamba;_Em (with DNA) = 350/461 nm
 
• Pale yellow solid soluble in water up to 2% (w/v)
 
• Store at 4°C and protect from light
 
• C₂₇H₃₁Cl₃N₆O.3H₂O
 
• MW: 615.98
 
• [23491-52-3]