DiSC2(5) (3, 3'-diethylthiadicarboCyanine, iodide)

DiSC₂(5) (3,3'-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization.

• &lamba;_Abs (pH 7.5 buffer) = 646 nm (free dye); ~586 nm (dsDNA); ~531 nm (PNA-DNA)
• Dark solid soluble in DMSO or MeOH
• Store at 4°C
• C₂₃H₂₃IN₂S₂
• MW: 518.48