DAPI

• Data sheet: View or download
• MSDS: View or download

DAPI (4',6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Also see DAPI dilactate, a more water soluble form of the dye. 

 
• Classic blue nuclear counterstain for fixed cells
 
• Can be used at higher concentrations to stain live cells
 
• &lamba;_Ex/&lamba;_Em = 358/461 nm (with DNA)
 
• Off-white solid soluble in H₂O
 
• Store at 4°C and protect from light, especially in solution
 
• C₁₆H₁₇Cl₂N₅
 
• MW = 350.25
 
• [28718-90-3]