10-Acetyl-3, 7-dihydroxyphenoxazine

10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), which has been marketed under the name Amplex® Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H₂O₂(reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H₂O₂ is produced by many enzymatic reactions, and thereforeADHP can be used to detect the activity of many different enzymes.

• Detect HRP in immunoassays
• Detect trace amounts of hydrogen peroxide (H₂O₂)
• Detect using fluorometer or microplate reader
• λ_Ex/λ_Em(pH 9)= 571/585 nm (end product)
• ε= 54,000 (end product)
• White to off-white solid soluble in DMSO or DMF
• Store desiccated at -20°C and protect from light
• C₁₄H₁₁CNO₄
• MW: 257.24
• [119171-73-2]

• Data sheet: View or download