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Human NF-KappaB p65 Activity Assay Kit

Catalogue number:
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TFEH-p65-1
Supplier:
RayBiotech
Size:
1 plates
Product is available in:
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£687.00
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Sample Type:

Cell Lysates, Nuclear Extracts

Species Reactivity:

Human

Alternative Names:

Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)

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Product Description:

This assay uses a dsDNA coated plate with canonical NF-kB binding sequences to semi-quantitatively detect active NF-kB in lysates or nuclear extracts. Dry ice shipment (additional fee).

Figure 1: Transcription factor assay of
Figure 1: Transcription factor assay of p53 from nuclear extracts of HeLa cells or HeLa cells treated with NiCl2. After stimulation activated p53 binds with its corresponding DNA. A. Western-blot result of p53 from cytoplasm and nuclear fractions. B. Transcription factor assay of p53 from nuclear fractions with RayBio® p53 TF-Activity Assay Kit.
Figure 2: Transcription factor assay of
Figure 2: Transcription factor assay of p53 from nuclear extracts of HeLa cells or HeLa cells treated with NiCl2 with the specific competitor or non-specific competitor. The result shows specific binding of p53 to the p53 DNA binding site.