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Resorufin β-D-Galactopyranoside

Supplier:
Catalogue number:
ANA85617
Size:
25 mg
Product is available in:
  • UK
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Unlike FDG that requires a two-step hydrolysis to generate maximum fluorescence, resorufin β-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence. This substrate is especially useful for sensitiveEnzyme measurements in ELISAs. The relatively low pKa (~6.0) of resorufin (the enzymatic hydrolysis product of resorufin galactoside) with Ex/Em=573/585 nm permits continuous measurement of enzymatic activity. Resorufin galactoside has also been used to quantitate β-galactosidase activity in single yeast cells by flow cytometry and to detect immobilised β-galactosidase activity.

Product Type:

Reagents & Kit Components

Storage Temp:

-20°C desiccated and protected from light

Compound Purity:

Minimum Purity: 95%

Molecular Weight:

375.33

Reference:

1. Yang NC and Hu ML (2004). Anal Biochem 325, 337-43. 2. Chiocconi A, et al. (2000). Carbohydr Res 323, 7-13. 3. Hakamata W, et al. (2000). Carbohydr Res 324, 107-15.4. Weiss DJ, et al. (1997). Hum Gene Ther 8, 1545-54.5. Lukomskaya IS, et al. (1996). Clin Chim Acta 244, 145-54.6. Usui T, et al. (1992). Anal Biochem 202, 61-7.7. Cousin MA, et al. (1989). J Appl Bacteriol 66, 311-7.8. Bowen DM and Radin NS (1969). JNC 16, 457-460.

UNSPSC Code:

12352200

Reagents & Kit Components from AnaSpec: