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HIV-1 Protease, recombinant - ANA72028-5 - 5 µg - AnaSpec - proteins & peptides

HIV-1 Protease, recombinant
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Size: 5 µg
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HIV-1 Protease, recombinant

The 10-12 kD aspartic protease of human immunodeficiency virus-1 (HIV-1), active only when it forms a homodimer, is required for the post-translational cleavage of the precursor polyproteins, Gag, Gag-Pol, Pol, and Nef. Since the cleavages of precursors are essential for the maturation of HIV infectious particles, this protease is one of the key targets for developing anti-AIDS drugs. The recombinant HIV-1 protease (wild type, NCBI accession: NC_001802) contains the 99 amino acid sequence reported by Schneider, et al., with 6x His-tag at its C-terminal. 50-100 ng of HIV-1 protease is sufficient for FRET-based activity assays (SensoLyte® 490, 520, and 620 HIV Protease Assay Kits, AnaSpec Cat#71127, 71147, 71148) in high throughput drug screening.

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Proteins & Peptides
Seelmeier, S. et al., Proc. Natl. Acad .Sci.USA. 85, 6612-6616 (1988); Schneider, J. and S. B. Kent, Cell 54, 363-368 (1988).
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