HiLyte™ Fluor 488 hydroxylamine is a carbonyl-reactive labeling dye, which reacts more readily with aldehydes at physiological pH than other primary amine-containing reagents (such as hydrazides and amines). The reaction of HiLyte™ Fluor 488 hydroxylamine with aldehydes yields oxime ethers, which are stable at physiological conditions. Therefore, HiLyte™ Fluor 488 hydroxylamine can be used to directly detect aldehydes in proteins and DNA. Extinction Coefficient (M-1cm-1): 70,000 Fluorescence quantum yield: 0.91 Fluorescence Life Time (ns): 4.1
Proteins & Peptides
-20°C desiccated and protected from light
D. Boturyn, et al, Tetrahedron, 53, 5485 (1997); J. G. Hecker, et al, ChemmedChem, 3, 1356 (2008); Y. Zeng, et al, Nature methods, 6, 207 (2009).