Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
Reagents & Kit Components
-20°C desiccated and protected from light
Biswas S, et al. (2004). Quenching of fluorescence of 1-hydroxypyrene-3,6,8-trisulfonate (HPTS) by Cu2+, Co2+, Ni2+, I-, and cetylpyridinium (CP+) ions in water/AOT/heptane microemulsion. J Colloid Interface Sci 271, 157-62, Ma LY, et al. (2004). A long lifetime chemical sensor: study on fluorescence property of fluorescein isothiocyanate and preparation of pH chemical sensor. Spectrochim Acta A Mol Biomol Spectrosc 60, 1865-72, Kermis HR, et al. (2003). Rapid method for the preparation of a robust optical pH sensor. Analyst 128, 1181-6, Kermis HR, et al. (2002). Dual excitation ratiometric fluorescent pH sensor for noninvasive bioprocess monitoring: development and application. Biotechnol Prog 18, 1047-53, Ozkan P and Mutharasan R (2002). A rapid method for measuring intracellular pH using BCECF-AM. Biochim Biophys Acta 1572, 143-8, Marechal X, et al. (1999). In vivo application of intestinal pH measurement using 2