Fluorescein *Fluorescence Reference Standard*
pH-dependent fluorescence; Maximum intensity observed in basic solutions.
Reagents & Kit Components
-20°C desiccated and protected from light
332.3
Biswas S, et al. (2004). Quenching of fluorescence of 1-hydroxypyrene-3,6,8-trisulfonate (HPTS) by Cu2+, Co2+, Ni2+, I-, and cetylpyridinium (CP+) ions in water/AOT/heptane microemulsion. J Colloid Interface Sci 271, 157-62, Ma LY, et al. (2004). A long lifetime chemical sensor: study on fluorescence property of fluorescein isothiocyanate and preparation of pH chemical sensor. Spectrochim Acta A Mol Biomol Spectrosc 60, 1865-72, Kermis HR, et al. (2003). Rapid method for the preparation of a robust optical pH sensor. Analyst 128, 1181-6, Kermis HR, et al. (2002). Dual excitation ratiometric fluorescent pH sensor for noninvasive bioprocess monitoring: development and application. Biotechnol Prog 18, 1047-53, Ozkan P and Mutharasan R (2002). A rapid method for measuring intracellular pH using BCECF-AM. Biochim Biophys Acta 1572, 143-8, Marechal X, et al. (1999). In vivo application of intestinal pH measurement using 2'''',7''''-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence imaging. Photochem Photobiol 70, 813-9, Weinlich M, et al. (1998). Simultaneous detection of cell volume and intracellular pH in isolated rat duodenal cells by confocal microscopy and BCECF. Res Exp Med (Berl) 198, 73-82, Boyarsky G, et al. (1996). Inadequacy of high K+/nigericin for calibrating BCECF. I. Estimating steady-state intracellular pH. Am J Physiol 271, C1131-45, Boyarsky G, et al. (1996). Inadequacy of high K+/nigericin for calibrating BCECF. II. Intracellular pH dependence of the correction. Am J Physiol 271, C1146-56, Carrero J and Voss EW, Jr. (1996). Temperature and pH dependence of fluorescein binding within the monoclonal antibody 9-40 active site as monitored by hydrostatic pressure. J Biol Chem 271, 5332-7.
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