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HCV Protease FRET SubstRate (RET S1) - ANA22991-025 - 0.25 mg - AnaSpec - proteins & peptides

HCV Protease FRET SubstRate (RET S1)
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Size: 0.25 mg
£233.00
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Catalogue number:
ANA22991-025
Name:
HCV Protease FRET SubstRate (RET S1)
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This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.

Data sheet:

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Product Type:
Proteins & Peptides
Alternative Names:
Ac-DE-D(Edans)-EE-Abu-ψ-[COO]-AS-K(Dabcyl)-NH2
Storage Temp:
-20°C
Molecular Weight:
1548.6
Reference:
Lin, C. et al. J. Biol. Chem. 10, 1074 (2004); Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999); Liu, Y. et al. Anal. Biochem. 267, 331 (1999); Taliani, M. et al. Anal. Biochem. 240, 60 (1996).
UNSPSC Code:
12352202