*

HCV Protease FRET Substrate (RET S1)

Supplier:
Catalogue number:
ANA22991-025
Size:
0.25 mg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£264.00 Shipping is calculated in checkout

This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to theEnzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.

Product Type:

Proteins & Peptides

Storage Temp:

-20°C

Compound Purity:

Peak Area by HPLC ≥95%

Molecular Weight:

1548.6

Reference:

Lin, C. et al. J. Biol. Chem. 10, 1074 (2004); Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999); Liu, Y. et al. Anal. Biochem. 267, 331 (1999); Taliani, M. et al. Anal. Biochem. 240, 60 (1996).

UNSPSC Code:

12352202

Sorry - there are no images available for this product.