β-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit
Catalogue number:
CAY700740-96 wells
Supplier:
Size:
96 wells _$$_
Product is available in:
£444.00
Estimated delivery Fri 24 May
Shipping is calculated in checkout
Shipping is calculated in checkout
Product Description:
Cayman’s β-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying β-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for β-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The fluorescence is directly proportional to the β-HB concentration.
Storage Temperature:
-20°C
