ITSX Supplement (ITS-X) (100X)

ITSX Supplement (100X) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) used to culture cells. ITS-X solution is routinely used, in combination with 2 to 4% FBS, to support cell types that require ethanolamine, such as bronchial epithelial cells. This contains Insulin (1mg/ml), Transferrin (0.55mg/ml) and Sodium selenite (0.67mg/L). Produced in Earle's Balanced Salt Solution (EBSS). Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite is a co-factor for glutathione peroxidase and other proteins that is used as an anti-oxidant in media. Ethanolamine is a precursor of phospho-glycerides which are essential to the structure of the plasma membrane and cellular organelles. Solution is a clear, colorless solution that is free of particulates. Synonyms: Insulin-Transferrin-Selenium-Ethanolamine; ITS -X; ITS; Insulin Transferrin Selenite Quality Control: Tested for the ability to support the proliferation of VERO cells in serum free conditions over a 3 day time period. pH: 7.8-8.2 Endotoxin: ≤1EU/mg Storage and Stability: Store at 4°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.