LipidLaunch™ LNP Apparent pKa Assay Kit (TNS Method)

Cayman’s LipidLaunch™ LNP Apparent pKa Assay Kit (TNS Method) provides a robust and easy-to-use platform for testing the apparent/global pKa of formulated LNPs. The assay uses a fluorogenic probe, 6-(p-toluidino)-2-naphthalenesulfonic acid sodium salt (TNS). This probe fluoresces in association with positively charged membranes, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 320 and 450 nm, respectively. TNS loses fluorescence as it dissociates, so a titration of the pH in the presence of LNPs results in a curve, with the 50% maximal fluorescence point called the apparent pKa of the particle.