www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

loading...
loading...
  1. home
  2. Absea
  3. BLNK Antibody
*

BLNK Antibody

Catalogue number:
Add to favourites
KC-2063-100
Supplier:
Absea
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£306.00
add to basket
Shipping is calculated in checkout
Applications:

IHC / ICC

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Recombinant human BLNK

Alternative Names:

B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65

Product Description:

BLNK Antibody - 100 µl

IHC-P analysis of tonsil tissue by BLNK
IHC-P analysis of tonsil tissue by BLNK antibody (K1E042_19B6). IHC-P was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with BLNK antibody (K1E042_19B6) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm.