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ACE2 Antibody

Catalogue number:
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KC-2003-100
Supplier:
Absea
Size:
100 µl
Product is available in:
  • UK
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£306.00
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Applications:

IHC / ICC

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Recombinant human ACE2

Alternative Names:

Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, ACEH, Angiotensin-converting enzyme-related carboxypeptidase, ACE-related carboxypeptidase, Metalloprotease MPROT15) [Cleaved into: Processed angiotensin-converting enzyme 2]

Product Description:

ACE2 Antibody - 100 µl

IHC-P analysis of kidney tissue by ACE2
IHC-P analysis of kidney tissue by ACE2 antibody (K92008_5F8). IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with ACE2 antibody (K92008_5F8) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Microvillus of cells in tubules are positively stained.