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HMOX2 Antibody

Catalogue number:
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KC-2002-100
Supplier:
Absea
Size:
100 µl
Product is available in:
  • UK
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£306.00
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Applications:

IHC / ICC

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Recombinant human HMOX2

Alternative Names:

Heme oxygenase 2, HO-2) [Cleaved into: Heme oxygenase 2 soluble form]

Product Description:

HMOX2 Antibody - 100 µl

IHC-P analysis of testis tissue by HMOX2
IHC-P analysis of testis tissue by HMOX2 antibody (K56012_8C10). IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with HMOX2 antibody (K56012_8C10) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Cells in seminiferous ducts are positively stained at the cytoplasm.