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SH3RF1 Antibody

Catalogue number:
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KC-1784-100
Supplier:
Absea
Size:
100 µl
Product is available in:
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£344.00
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Applications:

IP&WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

Immunogen:

Recombinant human SH3RF1

Alternative Names:

E3 ubiquitin-protein ligase SH3RF1, Plenty of SH3s, Protein POSH, RING finger protein 142, RING-type E3 ubiquitin transferase SH3RF1, SH3 domain-containing RING finger protein 1, SH3 multiple domains protein 2

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Product Description:

SH3RF1 Antibody - 100 µl

15 µg of HeLa lysate and 15 µg of mouse
15 µg of HeLa lysate and 15 µg of mouse brain tissue lysate were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K1E030_5E8 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. SH3RF1 band was visualized using ECL Western Blotting Substrate. Lane 1: 15 µg of HeLa lysate Lane 2: 15 µg of mouse brain tissue lysate Result: K1E030_5E8 can detect SH3RF1 by Western blotting.
Immunoprecipitation was performed by inc
Immunoprecipitation was performed by incubation of 2.5 µg of K1E030_5E8 with mouse brain tissue lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. SH3RF1 (K1E030_5E8) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Mouse brain tissue lysate Lane 2: SH3RF1 immunoprecipitated from mouse brain tissue lysate by K1E030_5E8 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K1E030_5E8 can immunoprecipitate SH3RF1.