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NKX2-1 Antibody

Catalogue number:
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KC-1139-100
Supplier:
Absea
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£306.00
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Applications:

IHC / ICC

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Immunogen:

Recombinant human NKX2-1

Alternative Names:

Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1, Thyroid-specific enhancer-binding protein, T/EBP

Product Description:

NKX2-1 Antibody - 100 µl

IHC-P analysis of lung tissue by NKX2-1
IHC-P analysis of lung tissue by NKX2-1 antibody (K52012_7B1). IHC-P was performed using sections of the formalin-fixed paraffin-embedded lung tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NKX2-1 antibody (K52012_7B1) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Alveolar cells are positively stained at the nuclei.