Human papillomavirus (HPV) is a common sexually transmitted infection (STI), which is related to several diseases, such as genital warts and tumours. The virus has no envelope and uses major capsid protein (L1) and Minor capsid protein (L2) as its capsid with a double-stranded circular DNA inside. HPV (51) Fluc Pseudovirus is packaged with L1 and L2 proteins of HPV type 51 expressed in SV40 Large T antigen overexpressing HEK 293T cells, as well as a double-stranded DNA containing firefly luciferase gene. The pseudovirus can mimic the structure of HPV, while does not contain oncogenes from HPV, such as HPV E6 / E7, and replication deficient. It can effectively infect SV40 Large T antigen overexpressing HEK 293T cells and can be used in determining neutralizing antibody titer, screening for inhibitors, studying virus invasion and HPV vaccine development. The reference sequences are: NCBI # ACV88631.1 (L1) and NCBI # AZS64278.1 (L2), mutation compared to L2 (Accession # AZS64278.1): K284T.
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