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Goat F(ab')2 Anti-Human IgM-UNLB

Catalogue number:
Supplier:
Size:
0.5 mg
Product is available in:
  • UK
  • Ireland
  • Europe
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Applications:

ELISA, FC, ICC, ELISPOT, Stim

Antibody Host:

Goat

Species Reactivity:

Human

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Human IgM

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Product Description:

Pepsin digest of Goat Anti-Human IgM (SouthernBiotech Cat. No. 2020) - Pooled antisera from goats hyperimmunized with human IgM; purified by affinity chromatography on human IgM covalently linked to agarose; reacts with the heavy chain of human IgM; cross-adsorbed against human IgG and IgA for minimal reactivity

Ramos cell lysates treated with HM71224
Ramos cell lysates treated with HM71224 and stimulated with Goat F(ab’)2 Anti-Human IgM-UNLB (SB Cat. No. 2022-01) were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-phospho-PLCγ2Y1217 followed by an HRP conjugated secondary antibody and chemiluminescent detection. Image from Kim Y, Park KT, Jang SY, Lee KH, Byun J, Suh KH, et al. HM71224, a selective Bruton's tyrosine kinase inhibitor, attenuates the development of murine lupus. Arthritis Res Ther. 2017;19:211. Figure 1(a)
ELISA plate was coated with purified hum
ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human IgM-UNLB (SB Cat. No. 2022-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).