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ERK2 (MAPK1) Mouse Monoclonal Antibody [Clone ID: OTI1C11]

Catalogue number:
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TA500474
Supplier:
OriGene
Size:
100 µl
£485.00
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This product is no longer available to order.
Applications:

IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI1C11 (formerly 1C11)

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1. Positive lysates LY408481 (100ug) and LC408481 (20ug) can be purchased separately from OriGene.
Immunoprecipitation of MAPK1 by using Tr
Immunoprecipitation of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibody (Negative control: IP without adding anti-MAPK1 antibody). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant-MAPK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.