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  3. anti-CD3 zeta phospho (Tyr142) antibody [EM-54]
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anti-CD3 zeta phospho (Tyr142) antibody [EM-54]

Catalogue number:
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ARG65722-100ug
Supplier:
Arigo Biolaboratories
Size:
100 µg
Product is available in:
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£502.00
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Applications:

Flow cytometry, Western blot

Antibody Host:

Mouse

Target:

CD3 zeta

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Phosphospecific peptide around Tyr142 of Human CD3 zeta.

Alternative Names:

IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247

Product Description:

Mouse Monoclonal antibody [EM-54] recognizes CD3 zeta phospho (Tyr142)

Westren blot: Cell lyzates of HEK293T/17
Westren blot: Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were stained with ARG65722 anti-CD3 zeta phospho (Tyr142) antibody [EM-54]. Lanes:1) Wt mCD3zeta pervanadate non-stimulated2) Wt mCD3zeta pervanadate stimulated3) mut. mCD3zeta Y/F2-64) mut. mCD3zeta Y/F1 and 3-65) mut. mCD3zeta Y/F1-2 and 4-66) mut. mCD3zeta Y/F1-3 and 5-67) mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native)8) mut. mCD3zeta Y/F1-5.