RNases are a class of hydrolytic enzymes that catalyzes both the in vivo and in vitro degradation of ribonucleic acid (RNA) molecules into smaller components. RNase enzymes are categorized into two groups: exoribonucleases and endoribonucleases. RNases are ubiquitous in the environment, and in some biological materials, they are present in relatively high concentrations. RNases also frequently contaminate common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storage. It is often used DEPC and/or heaten to remove RNases from containers in experiments. Since even only minute amounts of RNase contamination would ruin the experiment, it is necessary to evaluate the presence of RNase with the RNase Activity Assay Kit (Fluorescence).
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