www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

CRISPRa (SAM) Jurkat Cell Line

Catalogue number:
Supplier:
Size:
2 vials
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£2660.00 Shipping is calculated in checkout
Alternative Names:

Synergistic Activation Mediator cells, dCas9 cell pool

Product Description:

This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2-tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene. This product is for research use only and is subject to a Limited Use License. For your order to be processed, you will be required to complete a Limited Use License Agreement form at: https://bpsbioscience.com/license/agreement/form, ensuring you state your Purchase Order number when asked for Sales Order/Quote #. Further information can be found at: https://bpsbioscience.com/license.

CRISPRa (SAM) Jurkat Cell Pool
CRISPRa (SAM) Jurkat Cell Pool