PARP3 Homogenous Assay Kit
ADPRT3, Protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase diphtheria toxin-like 3, DNA ADP-ribosyltransferase PARP3, NAD(+) ADP-ribosyltransferase 3, Poly [ADP-ribose] polymerase 3, Poly[ADP-ribose] synthase 3
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The PARP3 Homogeneous Assay Kit is designed to measure PARP3 activity for screening and profiling applications. PARP3 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP3 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, a sample containing PARP3 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
