Recombinant Anti-TNF alpha Fab Antibody

Anti-TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNF is an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production) , cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD) . Anti-TNF alpha antibody, Adalimumab, a monoclonal antibody, working by inactivating TNFα is approved to treat rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, ulcerative colitis, psoriasis, hidradenitis suppurativa, uveitis, and juvenile idiopathic arthritis and sold under the brand name Humira. This antibody is made from a Adalimumab Fab fragment making it specific, efficient, and effective. Humanized Recombinant Anti-TNFα Fab fragment Antibody is useful for researchers interested in Cancer and Inflammatory disease research. Humanized Recombinant Anti-Anti-TNFα Fab fragment Antibody has been tested for use in Western Blot and ELISA. This antibody recognizes structured TNFα and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user.