www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

loading...
loading...
  1. home
  2. GenScript Biotech
  3. GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse
*

GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse

Catalogue number:
Add to favourites
A01936-40
Supplier:
GenScript Biotech
Size:
40 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£112.00
add to basket
Shipping is calculated in checkout

GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse - 40 µg

image 0
image 1
ELISA binding of GenCRISPRᵀᴹ SpCas9 Anti
ELISA binding of GenCRISPRᵀᴹ SpCas9 Antibody (14B6) (GenScript, A01936-40) with recombinant Streptococcus pyogenes CRISPR/Cas9 protein. Coating antigen: SpCas9, 1 µg/ml. SpCas9 antibody dilution start from 3000 ng/ml, EC50= 24.51 ng/ml.
Immunofluorescence staining of GenCRISPR
Immunofluorescence staining of GenCRISPRᵀᴹ SpCas9 Antibody (14B6) (GenScript, A01936-40) with SpCas9 in Hela cells. HeLa cells transfected with PX458 (pSpCas9(BB)-2A-GFP) (green) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min. The cells were then incubated with GenCRISPRᵀᴹ SpCas9 Antibody (14B6) (GenScript, A01936-40) at 2 μg/ml, 1 μg/ml and mouse IgG at 2 μg/ml, 1 μg/ml at room temperature for 2h, followed by a further incubation at 37℃ for 1h with Goat Anti-Mouse IgG Antibody (H&L) [ifluor 555], pAb (GenScript) (red) at 5 μg/ml. DAPI was used to stain the cell nuclei (blue) at a concentration of 0.2 μg/ml for 2h at room temperature.