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GenCRISPR™ SpCas9 Antibody (4A1), mAb, Mouse

Catalogue number:
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A01935-40
Supplier:
GenScript Biotech
Size:
40 µg
Product is available in:
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£116.00
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GenCRISPR™ SpCas9 Antibody (4A1), mAb, Mouse - 40 µg

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ELISA binding of GenCRISPRᵀᴹ SpCas9 Anti
ELISA binding of GenCRISPRᵀᴹ SpCas9 Antibody (4A1) (GenScript, A01935-40) with recombinant Streptococcus pyogenes CRISPR/Cas9 protein. Coating antigen: SpCas9, 1 µg/ml. SpCas9 antibody dilution start from 1000 ng/ml, EC₅₀= 9.17 ng/ml.
Immunofluorescence staining of GenCRISPR
Immunofluorescence staining of GenCRISPRᵀᴹ SpCas9 Antibody (4A1) (GenScript, A01935-40) with SpCas9 in Hela cells. HeLa cells transfected with PX458 (pSpCas9(BB)-2A-GFP) (green) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min. The cells were then incubated with GenCRISPRᵀᴹ SpCas9 Antibody (4A1) (GenScript, A01935-40) at 0.25 μg/ml, 0.5 μg/ml, 1 μg/ml and mouse IgG at 1 μg/ml at room temperature for 2h, followed by a further incubation at 37℃ for 1h with Goat Anti-Mouse IgG Antibody (H&L) [ifluor 555], pAb (GenScript) (red) at 5 μg/ml. DAPI was used to stain the cell nuclei (blue) at a concentration of 0.2 μg/ml for 2h at room temperature.