Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sen
Mouse
IgG1, k
Epitope Tag Antibodies
A synthetic peptide HHHHHH coupled to KLH
THE™ Anti-His mAb
- Data sheet: View or download
- MSDS: View or download
- Protocol: View or download
Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognise 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognised and bound by this antibody. THE™ His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognises native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THE™ His Tag mAb recognises His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THE™ His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
