The Zyppy™ Plasmid DNA Purification Kits from Zymo Research enable the simple and rapid purification of transfection-quality plasmid DNA directly from E. coli. Bypassing standard bacterial culture centrifugation and resuspension steps common to many plasmid preparation procedures, the high quality, endotoxin-free plasmid DNA is ideal for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing and other sensitive downstream applications.
Benefits of using the Zyppy Plasmid DNA Purification Kits
• High quality, endotoxin-free plasmid DNA isolated
• Fast protocol
• Simple procedure
• No cell pelleting
• No resuspension steps
• Easy visualisation of complete bacterial cell lysis and neutralisation using coloured buffers
• Suitable for all sensitive downstream applications including transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription and sequencing
Zyppy Plasmid DNA Purification Kits workflow
Zyppy Plasmid DNA Purification Kit comparisons
| Catalogue number | Name | Binding capacity | Elution volume | Culture input | Endotoxin levels | Format |
|---|---|---|---|---|---|---|
| D4036 D4019 D4020 D4037 |
Zyppy Plasmid Miniprep Kit | 25 µg | ≥ 30 µl | E.coli culture | ≤50 EU/µg DNA | Spin-column |
| D4041 D4042 D4043 |
Zyppy-96 Plasmid Kit | 5 µg | ≥ 30 µl | E.coli culture | ≤50 EU/µg DNA | 96-well plate |
| D4100 D4101 D4102 |
Zyppy-96 Plasmid MagBead Kit | 5 µg | ≥ 30 µl | E.coli culture | ≤50 EU/µg DNA | Magnetic beads |
Testimonials
“Performed side-by-side preparations of single culture split into two, using the Zyppy and a leading competitor's miniprep kit. The Zyppy kit saved me ~25 minutes and returned plasmid of better 260/280.”
Greg G., North Carolina State University
"The results gave what they promised! Clean DNA."
Pamela R., UT Medicine San Antonio
“It's so FAST! And it works!! I can't think of a way to improve this product. My big problem with it is that it is SO fast that I don't have time to label my tubes!”
Anita F., Fairfield University
Free samples
Request a free sample of the Zyppy Plasmid Miniprep Kit
Material available for download
Zyppy Plasmid Miniprep Kit protocol
Zyppy Plasmid Miniprep Kit datasheet
Zyppy-96 Plasmid Miniprep protocol
Zyppy-96 Plasmid Miniprep datasheet
Zyppy-96 Plasmid MagBead Miniprep protocol
Zyppy-96 Plasmid MagBead Miniprep datasheet
Zyppy citations
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| Zyppy™ Plasmid Miniprep Kit D4036 · Zymo Research | D4036 Zymo Research |
50 preps |
£89.00
50 preps
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| Zyppy™ Plasmid Miniprep Kit D4019 · Zymo Research | D4019 Zymo Research |
100 preps |
£156.00
100 preps
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| Zyppy™ Plasmid Miniprep Kit D4020 · Zymo Research | D4020 Zymo Research |
400 preps |
£513.00
400 preps
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| Zyppy-96™ Plasmid Kit D4042 · Zymo Research | D4042 Zymo Research |
4 x 96 preps |
£917.00
4 x 96 preps
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| Zyppy-96™ Plasmid Kit D4041 · Zymo Research | D4041 Zymo Research |
2 x 96 preps |
£531.00
2 x 96 preps
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| Zyppy-96™ Plasmid MagPrep Kit D4102 · Zymo Research | D4102 Zymo Research |
8 x 96 preps |
£1400.00
8 x 96 preps
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| Zyppy-96™ Plasmid MagPrep Kit D4101 · Zymo Research | D4101 Zymo Research |
4 x 96 preps |
£779.00
4 x 96 preps
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| Zyppy-96™ Plasmid Mag Preps Kit D4100 · Zymo Research | D4100 Zymo Research |
2 x 96 preps |
£449.00
2 x 96 preps
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| Zyppy™ Plasmid Miniprep Kit D4037 · Zymo Research | D4037 Zymo Research |
800 preps |
£892.00
800 preps
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| Zyppy-96™ Plasmid Kit D4043 · Zymo Research | D4043 Zymo Research |
8 x 96 preps |
£1639.00
8 x 96 preps
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