Zymo-Seq ATAC-Seq library preparation

Zymo-seq ATAC library kit

The Zymo-seq ATAC library preparation kit offers an all-in-one solution for library preparation from mammalian cell or tissue input. The streamlined workflow is capable of producing sequencing-ready libraries from as little as 50,000 cells in as little as 4 hours. The kit demonstrates outstanding consistency, producing highly correlated replicates from both fresh and frozen samples.

Benefits of the Zymo-seq ATAC library kit:

• Sequencing-ready libraries in as little as 4 hours
• Ready-to-use with a simplified workflow
• High reproducibility
• Designed to reduce mitochondrial contamination, saving reads for the sequences that matter and increasing read depth

Library preparation workflow

1. Cell lysis is gentle, ensuring mitochondrial DNA remains within the cell and doesn’t contaminate nuclei of interest.
2. A hyperactive Tn5 transposase tagged with Illumina-compatible adapter fragment and tags open chromatin sites.
3. The tagmented DNA is indexed and PCR amplified to create sequencing-ready libraries.

Material for download

Zymo-Seq ATAC library prep protocol

Zymo-Seq ATAC library prep datasheet